March 17, 1947
Mr. S. D. Beard
American Cyanamid Company
30 Rockefeller Plaza
New York, New York
Dear Mr. Beard:
Professor Campbell and Dr. Frank Lanni have recently been getting some very interesting results, from quantitative determinations of the amounts of precipitate formed when anti-SSI and anti-SSII is precipitated with the polysaccharides. They have asked me to write to you, in order to get some additional information.
The serum which has been used is anti-I and anti-II horse serum bled 1-11-40 and numbered 4616, which you gave to us. Could you send us information about the general immunizing schedule in the preparation of this serum? This is of interest because the experimental results which they have obtained indicate the presence of antibodies with combining groups of both kinds on the same molecule.
We have run short of specific substance SI, and need to have some more, preferably from one grams to five grams. It would be best to have Lot 230 if it is available. Dr. Piersma's letter of May 3, 1946 to me did not list type I pneumoccous polysaccharide, and so I am afraid that you may not have this material available for us.
Could you send us information about the general method used in the preparation of SII polysaccharide Lot 36T which was given to us.
Do you have available any house serum of type anti-I? Also any of type anti-II? Campbell and Lanni would like to make some comparative tests with a mixture of these two serums.
Also have you a preparation of the non-specific polysaccharide, the C-substance? A small amount of this would be very useful to us.
I am having a hard time now to get my work done in Pasadena and to arrange for my impending trip to Europe, for the months of June and July. These past few months have kept me very busy also because I am just publishing a textbook in freshman chemistry.
With the hope that I may see you again before too long, I am
cc: H. D. Piersma
D. H. Campbell
Dictated by Dr. Pauling but
signed in his absence